Evaluation of liquid biphasic Granada medium and instant liquid biphasic Granada medium for group B streptococcus detection

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Evaluation of liquid biphasic Granada medium and instant liquid biphasic Granada medium for group B streptococcus detection.

INTRODUCTION Group B streptococci (GBS) are transmitted from the mother to the newborn. Prevention of neonatal infection is achieved by intrapartum prophylaxis given to mothers colonized with GBS at 35 to 37 weeks of pregnancy. MATERIALS AND METHODS Liquid biphasic Granada medium (LB) and instant liquid biphasic Granada medium (ILB) were evaluated for GBS detection. Vaginal swabs obtained fro...

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Liquid Granada medium for detection of group B streptococci.

Heelan et al. (5) recently presented data on the sensitivity of a liquid Granada medium (9) (GBS broth) for detection of Streptococcus agalactiae. We have tested this medium, and it contains, as Granada medium, starch, proteose peptone 3, methotrexate, a Good’s buffer, serum, glucose, and selective agents. So, we agree with the authors that detection of orangered colonies in GBS broth is 100% s...

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Granada agar sensitivity and detection of group B streptococcus.

In their recent article Overman et al. (5) present data on the sensitivity of Granada agar (7). These data are similar to those presented by the same authors in a poster with the same title (D. D. Eley, S. B. Overman, B. E. Jacobs, and J. A. Ribes, Abstr. 100th Gen. Meet. Am. Soc. Microbiol., abstr. C-238, 2000). In the poster they reported a sensitivity of 60% and in the article they reported ...

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Evaluation of three commercial broth media for pigment detection and identification of a group B Streptococcus (Streptococcus agalactiae).

Detection of group B Streptococcus (GBS) strains at various bacterial concentrations was evaluated using three pigment-producing broth media. At 10(3) CFU/ml, StrepB carrot broth (SBCB), Granada instant liquid biphasic (IGLB), and Northeast Laboratory GBS screening medium (NEL-GBS) showed 100% detection, but at the lower bacterial counts, SBCB and IGLB were more sensitive than NEL-GBS after 24 h.

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Evaluation of a biphasic medium for blood cultures.

A study comparing the recovery of microorganisms from a transiently vented biphasic brain heart infusion medium bottle and a vacuum bottle containing tryptic soy broth demonstrated that growth was initially detected on the slant of the biphasic bottle or on a routine subculture of either broth in nearly 50% of instances. All organisms were isolated equally well in both bottles, with the excepti...

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ژورنال

عنوان ژورنال: Enfermedades Infecciosas y Microbiología Clínica

سال: 2008

ISSN: 0213-005X

DOI: 10.1157/13115539